Ultrafast LC-MS/MS analysis of 5-hydroxyindoleacetic acid (5-HIAA) in serum

Psychopathology and 5-hydroxyindoleacetic acid excretion.

CONSIDERABLE indirect evidence, associating serotonin with central nervous system function and the actions of important psychotropic drugs, led to suggestions192 of a relationship between serotonin metabolism and schizophrenia. Despite considerable effort, experimental support for this suggestion has been inconclusive. Among studies in this area are those on quantitative excretion, and variabil...

Cerebrospinal homovanillic acid and 5-hydroxyindoleacetic acid in huntington's chorea.

Acetic acid interference with determination of 5-hydroxyindoleacetic acid obviated.

1. Pratt, J. J., Steroid immunoassay in clinical chemistry. Clin. Chem. 24, 1869 (1978). 2. Shackleton, C. H. L., and Whitney, J. 0., Use of Sep-pak cartridges for urinary steroid extraction: Evaluation of the method for use prior to gas chromatographic analysis. Clin. Chim. Acta (in press). 3. Thenot, J.-P., and Horning, E. C., MOTMS derivatives of human steroids for GC and GC-MS studies. Anal...

The incidence of elevations in urine 5-hydroxyindoleacetic acid.

A 24-hour urine collection for 5-hydroxyindoleacetic acid (HIAA) is commonly performed to evaluate patients with suspected carcinoid syndrome. However, carcinoids are rare, and elevated results are common even when using an analytically specific method. To characterize this problem, the incidence of elevated results was examined in a population of 947 patient specimens received in a clinical re...

Specific photometric determination of 5-hydroxyindoleacetic acid in urine.

Udenfriend ‘s nitrosonaphthol test for 5-hydroxyindoleacetic acid (5-HIAA) is subject to interference by urinary phenolic acids and drug metabolites. Studies in our laboratory have shown that the specificity and sensitivity of the nitrosonaphthol reaction with 5-hydroxyindoles can be greatly enhanced by the addition of 2-mercaptoethanol. This observation serves as the basis of a simple, improve...